A putative probiotic isolated from hatchery reared juvenile Penaeus monodon

نویسندگان

  • Sijam Kamaruzaman
  • A. Christianus
  • M. Saleh Kamarudin
  • S. Shakibazadeh
چکیده مقاله:

 Probiotic is considered as a remedy to solve disease problem in shrimp aquaculture. Shrimp body and environment bacterial flora are recommended as reliable source for extracting of beneficial bacteria. Therefore, 5000 pieces of post larvae of Penaeus monodon were distributed equally in 4 tanks in probiotic and antibiotic free condition up to juvenile. Then microbiological samples from digestive system, muscles, body surface, rearing water and tank sediment were inoculated onto the selective media plates. Incident of total bacteria, Vibrio, Pseudomonas and Entrobacteriaceae were evaluated in different body parts and rearing environment of shrimp. The highest bacteria count for total bacteria, Vibrio, Pseudomonas was occurred in sediment followed by digestive system. No Entrobacteriaceae was observed from all samples. Isolated bacteria were identified and placed in 7 genera consisted of Vibrio, Shewanella, Burkholderia, Clavibacter, Staphylococcus, Brevibacterium, Corynebacterium, while one genus remained unidentified. Genus Shewanella exhibited highest frequent bacteria in digestive system of P. monodon which may be indicated its ability to colonization in digestive system. Shewanella algae was selected from whole isolated bacteria as candidate probiotic via well plate diffusion technique and cross streak antagonistic test against Vibrio harveyi, V. parahaemolyticus and V. alginolyticus. It was able to digest lipid and protein in tributyrin agar and skim milk agar.  

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A Circo-Like Virus Isolated from Penaeus monodon Shrimps

A virus with a circular Rep-encoding single-stranded DNA (ssDNA) (CRESS-DNA) genome (PmCV-1) was isolated from Penaeus monodon shrimps in Vietnam. The gene structure of the 1,777-nucleotide (nt) genome was similar to that of circoviruses and cycloviruses, but the nucleic acid and protein sequence identities to these viruses were very low.

متن کامل

Probiotic effect of Lactobacillus acidophilus against vibriosis in juvenile shrimp (Penaeus monodon)

The application of effective probiotics in shrimp aquaculture is an excellent alternative for chemicals and antibiotics to prevent disease control. This study was carried out to evaluate the probiotic potential of Lactobacillus acidophilus 04 (home made curd isolate) on pathogenic Vibrio in shrimp Penaeus monodon juveniles. L. acidophilus showed antibacterial activity against Vibrio parahaemoly...

متن کامل

Unexplained Mortalities of Hatchery-reared, Juvenile Oysters, Crassostrea Virginica (gmelin)

Survival, growth and pathology of juvenile oysters, Crassostrea virginica, in off-bottom culture at Oyster Bay and Fishers Island, New York, were monitored during the summer of 1991 to document and help explain the episodic mass mortalities of cultured seed oysters that have occurred in the northeastern USA over the past several years. At Oyster Bay, where the more detailed study was conducted,...

متن کامل

Feeding performance of juvenile hatchery-reared spotted seatrout Cynoscion nebulosus.

The feeding performance of individual hatchery-reared (HR) and wild juvenile spotted seatrout Cynoscion nebulosus was compared across a series of six 1·5 h feeding exposures over a 3 day period in a controlled experiment. The predation cycle served as a context for discerning feeding performance elements. The experimental design facilitated assessments of the effects of experience, motivation d...

متن کامل

Isolation, identification and molecular characterization of potential probiotic bacterium, Bacillus subtilis PPP 13 from Penaeus monodon

The beneficial effects of probiotics have been attributed to their ability to promote the immunological and nonimmunological defense barrier in the gut, normalization of increased intestinal permeability and altered gut microflora. Twelve different intestinal bacterial colonies were isolated from Black Tiger Shrimp (Penaeus monodon). Among them strain PPP 13 was studied and characterized due to...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 11  شماره 4

صفحات  849- 866

تاریخ انتشار 2012-10-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023